Journal: Journal of Clinical Microbiology

Article Title: Identification of Streptococcus sanguinis with a PCR-Generated Species-Specific DNA Probe

doi: 10.1128/jcm.41.8.3481-3486.2003

Figure Lengend Snippet: FIG. 1. AP-PCR fingerprint profiles generated from S. sanguinis, other oral mitis group species, and S. sanguinis clinical isolates. AP-PCR results were obtained by amplification of genomic DNA with primer OPA-02. A 1.6-kb amplicon was observed for all S. sanguinis strains. Other reference strains of the mitis group showed different AP-PCR patterns and the absence of a 1.6-kb amplicon. The non-S. sanguinis strains tested were as follows: 10557, S. oralis; 10558, S. gordonii; 15911, S. parasanguinis; 9811, S. oralis; 903, S. mitis; 49999, S. cristatus; WU2, S. pneumoniae; 35037, S. oralis.

Article Snippet: Probe SSA-1 (Fig. 3A) and probe SSA-3 (Fig. 3C) weakly hybridized to most of the Streptococcus species, including S. oralis (ATCC 10557), S. gordonii (ATCC 10558), S. oralis (ATCC 9811), S. parasanguinis (ATCC 15911), and S. vestibularis (ATCC 49124) but did not hybridize to actinomyces, lactobacilli, or E. coli.

Techniques: Generated, Amplification

Journal: Journal of Clinical Microbiology

Article Title: Identification of Streptococcus sanguinis with a PCR-Generated Species-Specific DNA Probe

doi: 10.1128/jcm.41.8.3481-3486.2003

Figure Lengend Snippet: FIG. 3. Dot blot hybridization shows the specificities of the three probes hybridized with different Streptococcus reference strains as well as with S. sanguinis clinical isolates. Probe SSA-2 was specific for type strain ATCC 10556 (B) and all S. sanguinis clinical isolates (D). SSA-1 and SSA-3 showed different degrees of hybridization to other species (A and C). The bacterial strains tested were as follows: 1, S. sanguinis; 2, S. oralis; 3, S. gordonii; 4, S. cristatus; 5, S. oralis; 6, S. mitis; 7, S. parasanguinis; 8, S. pneumoniae; 9, S. mutans; 10, S. salivarius; 11, S. sobrinus; 12, S. ratti; 13, S. vestibularis; 14, A. naeslundii; 15, L. acidophilus; 16, E. coli JM109.

Article Snippet: Probe SSA-1 (Fig. 3A) and probe SSA-3 (Fig. 3C) weakly hybridized to most of the Streptococcus species, including S. oralis (ATCC 10557), S. gordonii (ATCC 10558), S. oralis (ATCC 9811), S. parasanguinis (ATCC 15911), and S. vestibularis (ATCC 49124) but did not hybridize to actinomyces, lactobacilli, or E. coli.

Techniques: Dot Blot, Hybridization

Journal:

Article Title: A Streptococcal Adhesion System for Salivary Pellicle and Platelets

doi:

Figure Lengend Snippet: Adhesion phenotype

Article Snippet: Conversely, sHA adhesion-positive, platelet adhesion-negative strains also did not react with MAb 1.1. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Organism % Adhesion to: Reaction with MAb 1.1 ( A 405 ) c sHA a Platelets b S. sanguis 133-79 24.0 ± 0.9 59.0 ± 1.3 0.177 ± 0.036 S1219 27.4 ± 3.5 63.2 ± 4.5 0.205 ± 0.011 E1219 1.1 ± 0.02 7.5 ± 2.0 0 L74 16.0 ± 3.4 24.6 ± 10.3 0.104 ± 0.016 L59 18.5 ± 2.9 42.2 ± 1.3 0.135 ± 0.012 L14 22.3 ± 2.0 53.4 ± 2.1 0.165 ± 0.027 L52 19.0 ± 0.9 46.6 ± 13.8 0.167 ± 0.038 L22 20.7 ± 0.8 46.3 ± 4.7 0.127 ± 0.027 4124 23.5 ± 1.3 56.8 ± 3.0 0.229 ± 0.007 2017-78 3.8 ± 0.9 10.1 ± 3.9 0.075 ± 0.005 4123 0.5 ± 0.02 11.0 ± 3.0 0.043 ± 0.014 12 3.2 ± 0.4 16.7 ± 1.3 0.063 ± 0.018 12NA 4.4 ± 0.8 15.4 ± 0.2 0.070 ± 0.014 ATCC 10556 2.2 ± 0.2 2.8 ± 0.9 0.006 ± 0.006 804 1.4 ± 0.1 3.9 ± 1.1 0 HPC1 2.0 ± 0.2 2.9 ± 2.1 0 S. crista ATCC 51100 7.3 ± 0.9 11.0 ± 1.1 0.063 ± 0.017 ATCC 49999 6.6 ± 1.0 2.8 ± 0.4 0.037 ± 0.004 S. gordonii V288 14.2 ± 1.5 49.6 ± 4.9 0.163 ± 0.005 ATCC 10558 15.7 ± 1.6 36.5 ± 2.7 0.162 ± 0.025 ATCC 12396 22.0 ± 0.6 25.1 ± 4.2 0.109 ± 0.020 ATCC 33399 16.2 ± 1.0 3.0 ± 0.2 0.016 ± 0.005 Blackburn 26.1 ± 0.2 2.6 ± 0.1 0 S7 2.1 ± 0.1 4.5 ± 0.4 0 M5 4.1 ± 1.0 4.8 ± 0.6 0 L31 40.2 ± 8.2 14.9 ± 1.6 0.027 ± 0.026 S. mitis ATCC 903 0.7 ± 0.1 1.9 ± 0.4 0 S. mutans JBP 7.9 ± 0.6 34.1 ± 3.0 0.144 ± 0.023 Ingbritt 6.6 ± 1.3 5.8 ± 0.9 0 ATCC 25175 2.5 ± 0.03 7.6 ± 2.4 0.028 ± 0.026 ATCC 33402 0.2 ± 0.03 0 0.014 ± 0.007 ATCC 33535 2.5 ± 0.2 8.8 ± 2.6 0.058 ± 0.011 GS-5 0.4 ± 0.06 2.8 ± 1.8 0.015 ± 0.012 BHT 1.5 ± 0.1 4.4 ± 0.4 0.002 ± 0.026 S. oralis ATCC 10557 18.7 ± 0.7 21.6 ± 2.7 0.098 ± 0.014 9811 1.1 ± 0.1 5.5 ± 1.4 0 CR834 3.4 ± 0.1 3.4 ± 0.6 0.022 ± 0.02 L13 43.7 ± 7.6 17.1 ± 2.2 0.022 ± 0.014 S. parasanguis ATCC 15911 20.5 ± 5.4 5.6 ± 0.6 0.038 ± 0.011 ATCC 15912 0.7 ± 0.1 3.1 ± 1.0 0.003 ± 0.013 MGH145 0.4 ± 0.03 3.7 ± 0.6 0.016 ± 0.008 FW 213 1.3 ± 0.2 6.5 ± 1.4 0.077 ± 0.011 Open in a separate window a Values are expressed as means ± standard deviations ( n = 3). b Means of duplicate determinations with each of two platelet samples. c Values are expressed as means ± standard deviations ( n = 4).

Techniques:

Journal:

Article Title: A Streptococcal Adhesion System for Salivary Pellicle and Platelets

doi:

Figure Lengend Snippet: Inhibition of streptococcal adhesion to sHA and platelets by MAb 2.1

Article Snippet: Conversely, sHA adhesion-positive, platelet adhesion-negative strains also did not react with MAb 1.1. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Organism % Adhesion to: Reaction with MAb 1.1 ( A 405 ) c sHA a Platelets b S. sanguis 133-79 24.0 ± 0.9 59.0 ± 1.3 0.177 ± 0.036 S1219 27.4 ± 3.5 63.2 ± 4.5 0.205 ± 0.011 E1219 1.1 ± 0.02 7.5 ± 2.0 0 L74 16.0 ± 3.4 24.6 ± 10.3 0.104 ± 0.016 L59 18.5 ± 2.9 42.2 ± 1.3 0.135 ± 0.012 L14 22.3 ± 2.0 53.4 ± 2.1 0.165 ± 0.027 L52 19.0 ± 0.9 46.6 ± 13.8 0.167 ± 0.038 L22 20.7 ± 0.8 46.3 ± 4.7 0.127 ± 0.027 4124 23.5 ± 1.3 56.8 ± 3.0 0.229 ± 0.007 2017-78 3.8 ± 0.9 10.1 ± 3.9 0.075 ± 0.005 4123 0.5 ± 0.02 11.0 ± 3.0 0.043 ± 0.014 12 3.2 ± 0.4 16.7 ± 1.3 0.063 ± 0.018 12NA 4.4 ± 0.8 15.4 ± 0.2 0.070 ± 0.014 ATCC 10556 2.2 ± 0.2 2.8 ± 0.9 0.006 ± 0.006 804 1.4 ± 0.1 3.9 ± 1.1 0 HPC1 2.0 ± 0.2 2.9 ± 2.1 0 S. crista ATCC 51100 7.3 ± 0.9 11.0 ± 1.1 0.063 ± 0.017 ATCC 49999 6.6 ± 1.0 2.8 ± 0.4 0.037 ± 0.004 S. gordonii V288 14.2 ± 1.5 49.6 ± 4.9 0.163 ± 0.005 ATCC 10558 15.7 ± 1.6 36.5 ± 2.7 0.162 ± 0.025 ATCC 12396 22.0 ± 0.6 25.1 ± 4.2 0.109 ± 0.020 ATCC 33399 16.2 ± 1.0 3.0 ± 0.2 0.016 ± 0.005 Blackburn 26.1 ± 0.2 2.6 ± 0.1 0 S7 2.1 ± 0.1 4.5 ± 0.4 0 M5 4.1 ± 1.0 4.8 ± 0.6 0 L31 40.2 ± 8.2 14.9 ± 1.6 0.027 ± 0.026 S. mitis ATCC 903 0.7 ± 0.1 1.9 ± 0.4 0 S. mutans JBP 7.9 ± 0.6 34.1 ± 3.0 0.144 ± 0.023 Ingbritt 6.6 ± 1.3 5.8 ± 0.9 0 ATCC 25175 2.5 ± 0.03 7.6 ± 2.4 0.028 ± 0.026 ATCC 33402 0.2 ± 0.03 0 0.014 ± 0.007 ATCC 33535 2.5 ± 0.2 8.8 ± 2.6 0.058 ± 0.011 GS-5 0.4 ± 0.06 2.8 ± 1.8 0.015 ± 0.012 BHT 1.5 ± 0.1 4.4 ± 0.4 0.002 ± 0.026 S. oralis ATCC 10557 18.7 ± 0.7 21.6 ± 2.7 0.098 ± 0.014 9811 1.1 ± 0.1 5.5 ± 1.4 0 CR834 3.4 ± 0.1 3.4 ± 0.6 0.022 ± 0.02 L13 43.7 ± 7.6 17.1 ± 2.2 0.022 ± 0.014 S. parasanguis ATCC 15911 20.5 ± 5.4 5.6 ± 0.6 0.038 ± 0.011 ATCC 15912 0.7 ± 0.1 3.1 ± 1.0 0.003 ± 0.013 MGH145 0.4 ± 0.03 3.7 ± 0.6 0.016 ± 0.008 FW 213 1.3 ± 0.2 6.5 ± 1.4 0.077 ± 0.011 Open in a separate window a Values are expressed as means ± standard deviations ( n = 3). b Means of duplicate determinations with each of two platelet samples. c Values are expressed as means ± standard deviations ( n = 4).

Techniques: Inhibition

Journal: Medicine

Article Title: Ferroptosis-related gene signature predicts prognosis and immunotherapy response in hepatocellular carcinoma: A multi-cohort retrospective study

doi: 10.1097/MD.0000000000047262

Figure Lengend Snippet: Clinical validation of FRGs mRNA expression. (A–L) The mRNA expression levels of key genes HIF1A (A), SRC (B), EZH2 (C), AR (D), SLC1A5 (E), KIF20A (F), SLC7A11 (G), CARS1 (H), MYCN (I), PRDX6 (J), GPX4 (K), and KLF2 (L) were further validated using 30 clinical HCC samples * P < .05; ** P < .01; *** P < .001; **** P < .0001. AR = androgen receptor; ns: no statistical significance.

Article Snippet: The sections were incubated overnight at 4 °C with primary antibodies: SLC1A5 (1:500, 20350-1-AP, Proteintech), KIF20A (1:200, 15911-1-AP, Proteintech), SLC7A11 (1:200, 26864-1-AP, Proteintech), CARS1 (1:500, 15296-1-AP, Proteintech), MYCN (1:250, 10159-2-AP, Proteintech), PRDX6 (1:100, 13585-1-AP, Proteintech), GPX4 (1:2000, 67763-1-Ig, Proteintech), and KLF2 (1:200, bs-2772R, Bioss), and then with the secondary antibody for 1 hour at 24 °C.

Techniques: Biomarker Discovery, Expressing

Journal: Medicine

Article Title: Ferroptosis-related gene signature predicts prognosis and immunotherapy response in hepatocellular carcinoma: A multi-cohort retrospective study

doi: 10.1097/MD.0000000000047262

Figure Lengend Snippet: Clinical validations of FRGs protein expression. (A–H) Representative images of immunohistochemical staining for SLC1A5 (A), KIF20A (B), SLC7A11 (C), CARS1 (D), MYCN (E), PRDX6 (F), GPX4 (G), and KLF2 (H) in tumor and para-tumor tissues. Scale bars: 50 μm (main), 25 μm (insets). (I–P) Mean optical density (MOD) of FRGs proteins in tumor and para-tumor tissues, including SLC1A5 (I), KIF20A (J), SLC7A11 (K), CARS1 (L), MYCN (M), PRDX6 (N), GPX4 (O), and KLF2 (P). * P < .05; ** P < .01; **** P < .0001. ns = no statistical significance.

Article Snippet: The sections were incubated overnight at 4 °C with primary antibodies: SLC1A5 (1:500, 20350-1-AP, Proteintech), KIF20A (1:200, 15911-1-AP, Proteintech), SLC7A11 (1:200, 26864-1-AP, Proteintech), CARS1 (1:500, 15296-1-AP, Proteintech), MYCN (1:250, 10159-2-AP, Proteintech), PRDX6 (1:100, 13585-1-AP, Proteintech), GPX4 (1:2000, 67763-1-Ig, Proteintech), and KLF2 (1:200, bs-2772R, Bioss), and then with the secondary antibody for 1 hour at 24 °C.

Techniques: Expressing, Immunohistochemical staining, Staining